As one of the providers for ATAC-seq data analysis, CD Genomics uses bioinformatics to help you study the openness of chromatin accurately and efficiently. We provide convenient and fast biological data analysis services for scientific researchers all over the world.
Assay for transposase-accessible chromatin with high throughput sequencing (ATAC-seq) is a preferred technical method used to study the openness of chromatin. The principle is to use the transposase Tn5 to easily bind to open chromatin, but Tn5 does not have the cutting ability. ATAC-seq can completely capture the sequence of the entire region, and then sequence the DNA sequence captured by the Tn5 enzyme.
The sequencing linker is inserted into the open region of chromatin through the highly active Tn5 transposase, and the sequencing data can be obtained by high-throughput sequencing. Bioinformatics can be used to infer the accessibility of chromatin regions, to obtain information on all active transcriptional regulatory sequences in the whole genome in this space-time, to analyze transcription factor binding information, nucleosome region information, and distribution of transcriptional regulatory elements. The ATAC-Seq method requires fewer cells and the procedure is simple. It can detect the open state of chromatin throughout the genome.
Fig 1. Unique Chromatin Landscape in hSSCs Revealed by ATAC-Seq. (Guo J, et al. 2017)
Chromosomal structure research, Chromosome openness mapping, nucleosome region information, transcription factor binding site analysis, transcription regulatory element distribution.
Disease pathogenesis, pathogenesis of cancer and other diseases.
Potential biomarker prediction, clinical biomarkers, biomarker research.
Drug-related research, drug action mechanism, new drug development, drug resistance and sensitivity research.
Perfect analysis process, fast delivery and short cycle time
Rich and in-depth analysis content
Personalized analysis and chart making
Rich experience in bacterial genome analysis
CD Genomics has a complete, fast, efficient, sensitive and highly accurate ATAC-seq data analysis pipeline. We will provide you with reliable data analysis results.
|Data preprocessing||Data quality control|
|Remove low quality reads|
|Map to reference genome||Comparison rate|
|Unique comparison rate|
|Reads distribution statistics|
|Peak calling||Analysis of nucleosome and transcription factor binding sites|
|Peak position on chromosome|
|Peak length distribution|
|The distribution of peak on each genetic element|
|Differential peak analysis||Difference peak analysis|
|Difference peak gene annotation|
|GO enrichment analysis of related genes|
|KEGG pathway enrichment analysis of related genes|
For ATAC-seq data analysis, if you have other omics sequencing data, such as RNA and WGBS sequencing data, we can provide you with multi-omics association analysis according to you data. In addition, if custom analysis service is needed, please contact our technical support for an evaluation. We will design an analysis plan for you based on your data. For more information, please click online inquiry.
An approximately one to two weeks will be taken from obtaining the original data to reporting the result, but the specific analysis time is also related to the content of the analysis. If multi-group joint analysis is needed, a longer analysis cycle will be required.
CD Genomics has successfully conducted ATAC-seq data analysis on a variety of species, and samples can be tissue, cell or blood. Combined with rich project experience, strict data quality control and professional analysis process, we will provide you with the best data analysis services. Please contact us for more information and a detailed quote.