Regulation of gene expression is associated with open sites and the accessibility of the genome. Mutations in chromatin remodeling factors are associated with multiple aspects of human health. CD Genomics reveals and infers genome-wide chromatin changes as well as chromatin accessibility characteristics by combining epigenomic and transcriptomic sequencing data, or other databases.
Chromatin accessibility refers to the extent to which nuclear macromolecules can physically contact achromatized DNA, as determined by the occupancy and topology of nucleosomes and other chromatin binding factors that impede access to DNA. "Compact" structure prevents efficient transcription of target genes. Thus, the degree of structural "openness" of chromatin represents the degree of accessibility.
Our bioinformatics services are designed to bridge the limitations of current genome-wide chromatin accessibility data analysis and help clients derive more epistemic regulatory mechanisms from raw data to develop a wide range of applications, including cell proliferation research, functional diversification, and disease development research, etc.
Assay for transposase-accessible chromatin with high throughput sequencing (ATAC-seq) uses Tn5 transposase to obtain "open" chromatin DNA fragments, which are then sequenced and analyzed for deeper investigation of chromatin conformational changes.
Chromatin immunoprecipitation with NGS (ChIP-seq) is the core method for epigenomic studies. It uses specific antibodies to selectively enrich chromatin fractions containing specific DNA-binding proteins or histone modifications for genome-wide analysis.
Hi-C Seq uses restriction enzymes for restriction digestion of the genome and is able to score the contact frequency between almost any pair of genomic loci, examining genomic 3D organization at multiple scales.
CD Genomics is a professional bioinformatics service provider with years of experience in NGS and long read sequencing (PacBio SMRT and Oxford Nanopore platforms) data analysis, integrated analysis services, database construction and other bioinformatics solutions.