CD Genomics uses bioinformatics to provide metatranscriptome sequencing data analysis that can analyze the dynamic changes of microbial flora comprehensively. Our high-quality data analysis platform will be used to generate high-quality analysis reports in a fast analysis cycle.
Introduction to Metatranscriptome Analysis
Metatranscriptome sequencing is a new sequencing technology that performs high-throughput sequencing on the RNA of all microorganisms in a sample in a specific period and a specific environment, and directly obtains the information of all microorganisms in that environment. Compared with metagenome, metatranscriptome contains not only microbial species information, but also microbial gene expression information. Thus, the complex microbial community changes can be studied from the transcription level, and potential new genes can be better tapped.
Information mining of metatranscriptome data is carried out by bioinformatics, and the microbial species, genes, pathways and other information are analyzed from the G-based high-throughput sequencing data. It is possible to study the transcription of the entire genome of a group of microorganisms and the regulation of transcription regulation in a specific environment and a certain period of time, which can help to mine the functional genes of microorganisms and explore the mechanism of the relationship between microorganisms and the environment, diseases, animals and plants.
Fig 1. Metabolic circuit map constructed from the cryptophyte subset of the metatranscriptome. (Qiu D, et al. 2016)
Application Field
Metatranscriptome sequencing data analysis can be used for but not limited to the following microorganisms. For other type of microorganisms, Please contact us.
Soil microorganisms
Silt microorganism
Oral microorganisms
Marine microorganisms
Sewage microorganisms
Gut microbes
Fecal microorganisms
Microbes in extreme environments
Advantages of CD Genomics
A complete and highly accurate analysis pipeline
An experienced analytical team.
Strict data quality control.
Reliable analysis results.
Fast analysis cycle.
CD Genomics Data Analysis Pipeline
Bioinformatics Analysis Content
CD Genomics has a complete, efficient, sensitive and highly accurate metatranscriptome analysis pipeline. In addition to gene expression analysis and differential gene expression analysis, we can also perform species annotation and classification analysis, comparative analysis between multiple samples, etc.
| Data preprocessing |
Data quality control |
| Data statistics |
| Map microbial genomes |
| Map housekeeping gene |
| Map nr database |
| Clean rRNA |
| Transcript assembly |
Non-redundant transcript |
| Cluster analysis |
| Gene structure prediction |
| Unigene analysis |
| Function annotation |
KEGG annotation |
| Pfam annotation |
| COG annotation |
| GO annotation |
| Swissprot annotation |
| Gene expression analysis |
Gene differential expression analysis |
| GO enrichment analysis of differentially expressed genes |
| KEGG enrichment analysis of differentially expressed genes |
For macro transcriptome sequencing data analysis and other personalized analysis needs, please contact us by using the online inquiry and we will provide you with a reasonable analysis plan.
How It Works
CD Genomics is a professional bioinformatics service provider with years of experience in NGS and long read sequencing (PacBio SMRT and Oxford Nanopore platforms) data analysis, integrated analysis services, database construction and other bioinformatics solutions.
CD Genomics has successfully conducted metatranscriptome sequencing data analysis on a variety of microorganisms. If you are interested in our services, please contact us for more detailed information, and a CD Genomics representative is ready to answer your questions and get a complete understanding of your needs.
Reference
- Qiu D, et al. Cryptophyte farming by symbiotic ciliate host detected in situ[J]. Proceedings of the National Academy of Sciences, 2016:201612483.
