CD Genomics, as one of the most competitive service providers in sequencing and data analysis, we use CRISPR technologies and advanced bioinformatics to help you explore the genetic information and decipher the essence of life. Our skills and experience in data analysis can meet customers' personalized data analysis needs.
Introduction of CRISPR Validation Sequencing Data Analysis
In the rapidly evolving field of genomics, CRISPR-Cas9 technology has emerged as a breakthrough tool for precise genome editing. This revolutionary system enables targeted modification of DNA sequences and has great potential for applications in various fields such as biomedical research, agriculture and biotechnology. However, it is critical to ensure the accuracy and reliability of CRISPR experiments. This is where CRISPR validation sequencing data analysis comes into play. There are multiple methods to validate correct CRISPR gene editing and ensure that no unintended editing events have occurred against the target, including next-generation sequencing (NGS) of PCR amplicons via Sanger direct DNA sequencing, T7 nucleic acid endonuclease 1 (T7E1) mismatch detection analysis, tracking insertional deletions by disassembly (TIDE) analysis, and detection of insertional deletions by amplicon analysis (IDAA), etc.
Fig. 1. Validation of CRISPR/Cas9 cleavage activity in marmoset embryo. (Kumita W, et al, 2019)
The Bioinformatics Analysis That We Can Provide
Validation of CRISPR experiments is essential to determine the validity and specificity of genome editing. Misinterpretation of results or oversight of validation can lead to erroneous conclusions and impede progress in various research areas. CD Genomics provides professional services to provide accurate and reliable analysis of CRISPR validation sequencing data to customers worldwide. Our CRISPR validation sequencing data analysis services provide comprehensive solutions tailored to meet the specific needs of biologists.
CD Genomics leverages its expertise in genomics and bioinformatics to understand the potential of NGS in assessing CRISPR-Cas9-mediated modifications. We leverage cutting-edge NGS platforms and sophisticated bioinformatics pipelines and tools to process and analyze the vast amount of sequencing data generated during CRISPR validation. In addition, we integrate advanced technologies such as high-throughput sequencing, bioinformatics analysis, and functional assays to enable comprehensive validation of CRISPR sequencing data, including optimization of experimental protocols, control of off-target effects, and precise quantification of target editing efficiency.
Our Advantages:
We have the right experimental design, robust controls, and complementary validation techniques to ensure reliable CRISPR validation.
We are well versed in the intricacies of CRISPR validation and have the ability to address the challenges associated with analyzing sequencing data.
Our advanced next-generation sequencing (NGS) platform ensures high-throughput sequencing and accurate detection of CRISPR-Cas9-induced genomic modifications.
Whether it is target site identification, on-target editing efficiency analysis, detection of off-target effects, or characterization of insertional deletions and structural variants, our team can customize accurate and reliable CRISPR validation sequencing data analysis for your specific experiment.
We adhere to strict quality control measures throughout the CRISPR validation sequencing data analysis process to ensure accurate and reliable results.
Our CRISPR validation sequencing data analysis service provides comprehensive analysis and detailed reporting.
Bioinformatics Analysis Content:
|
Analysis/Pipeline |
Details |
| Identification and Characterization of Genomic Modifications |
Utilize machine learning, statistical modeling and data visualization techniques to accurately identify genomic modifications and assess their impact on gene function. |
| Validation of Targeted Editing Efficiency |
Use bioinformatics analysis to quantify the percentage of cells or alleles that have undergone the desired genomic modification. |
| Detection of off-Target Effects |
Advanced bioinformatics tools and algorithms are used to identify potential off-target loci and assess their editing efficiency. |
| Characterization of Insertion Deletions and Structural Variants |
Our bioinformatics analysis allows for accurate characterization of insertions, deletions and structural variants. |
| Gene Editing Specificity Analysis |
We utilize state-of-the-art software and algorithms to assess the overall specificity of CRISPR-Cas9-mediated genome editing. |
How It Works
CD Genomics is a high-tech company specializing in multiomic data analysis. We provide services such as project design, data analysis, and database construction. With a focus on developing breakthrough products and services, we are a pioneer in the biotechnology industry, serving researchers and partners worldwide.
CD Genomics has over a decade of experience in sequencing and analysis. We can help you with your project from experimental data, through all analyses to intact report at competitive rates. If you have any questions about how we can help you, please contact us. We look forward to working with you!
Reference
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Kumita W, Sato K, Suzuki Y, et al. Efficient generation of Knock-in/Knock-out marmoset embryo via CRISPR/Cas9 gene editing[J]. Scientific reports, 2019, 9(1): 1-13.
