Whole Genome De Novo Sequencing Data Analysis

Whole Genome De Novo Sequencing Data Analysis Online Inquiry

CD Genomics provides service on whole genome de novo sequencing data analysis. We use bioinformatics to help you explore the genome of new species. Our unique skills in data analysis can meet customers' personalized data analysis needs and provide the most comprehensive data analysis.

What Is De Novo Sequencing Data Analysis?

As its name implies, de novo sequencing data analysis analyzes the de novo sequencing data. de novo sequencing refers to sequencing a novel genome where there is no reference sequence available for alignment. Sequence reads are assembled as contigs, and the coverage quality of de novo sequence data depends on the size and continuity of the contigs (i.e., the number of gaps in the data).

A complete and accurate genome sequence is essential to the genomics study of new species and the investigation of complex structural genomic changes in wild relatives compared to published cultivar genome sequences.

With de novo sequencing data analysis, the first genome map for a species is generated, providing a valuable reference sequence for phylogenetic studies, analysis of species diversity, mapping of specific traits and genetic markers, and other genomics research.

Advantages of de novo Sequencing Data Analysis

◎ Generates accurate reference sequences, even for complex or polyploid genomes;
◎ Provides useful information for mapping genomes of novel organisms or finishing genomes of known organisms;
◎ Clarifies highly similar or repetitive regions for accurate de novo assembly;
◎ Identifies structural variants and complex rearrangements, such as deletions, inversions, or translocations.

CD Genomics Data Analysis Pipeline

Pipeline of de novo Sequencing Data Analysis - CD Genomics

Bioinformatics Analysis Content

Standard analysis content

Genome Survey

K-mer analysis and genome size estimation

Estimation of heterozygosity

Preliminary assembly

GC-Depth distribution analysis

Genome assembly

Assembly

GC-Depth distribution analysis

GC content distribution analysis

In-depth sequencing analysis

Assessment of autosomal area coverage

Assessment of gene area coverage (requires customer to provide EST or transcriptome sequence)

Genome annotation

repeat comment

Gene prediction

Gene function annotation

ncRNA annotation

Advanced analysis

Evolutionary analysis

Gene cluster analysis (also called gene family identification, animal TreeFam; plant OrthoMCL)

Species phylogenetic tree construction

Species divergence time estimation (requires calibration time information)

Genomic collinear analysis

Whole genome replication analysis (animal WGAC; plant WGD)

Customized Data Analysis

The customized information analysis content can be negotiated and determined according to the needs of customers.

Turnaround Time

Whole Genome De Novo Sequencing  Data Analysis

About one to two weeks, it’s related to the content of the project and the complexity of the genome and depending on the number of samples and the quality and size of the sample data. For more information, please contact us.

CD Genomics has over a decade of experience in whole genome de novo sequencing data analysis. We also have significant expertise in analyzing data from genomics, and other projects, to help drive research forward. We can help you with your project from experimental data, through all analyses and to result report, at competitive rates.If you have any questions about how we can help you, please get in touch. We look forward to working with you!

* For Research Use Only. Not for use in diagnostic procedures.
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