CD Genomics uses bioinformatics to help you perform reduced representation bisulfite sequencing (RRBS) data analysis accurately and efficiently. Our unique data analysis skills and advanced software tools can meet customers' personalized laboratory data analysis needs and provide the most easy-to-interpret data analysis reports.
DNA methylation refers to the covalent bonding of a methyl group at the 5'carbon position of the cytosine of genomic CpG dinucleotide under the action of DNA methylation transferase. Methylation is a form of chemical modification of DNA that changes genetic performance without changing the DNA sequence. It is one of the main mechanisms of epigenetic modification and has a fundamental impact on gene expression and cell activity.
Reduced representation bisulfite sequencing (RRBS) is a cost-effective method for methylation research. It uses restriction enzymes to digest the genome, which greatly improves the sequencing depth of the CpG region. Compared with whole-genome methylation sequencing data, the amount of data obtained from RRBS will be greatly reduced, but the resolution of CpG island, promoter region and enhancer element region will reach higher precision, thereby improving the efficiency of data analysis. RRBS has been widely used in the research of large-scale clinical samples.
Fig 1. The methylome in Acute Promyelocytic Leukemia. (Schoofs T,et al. 2013)
RRBS data analysis can be used for but is not limited to the following research:
Genetic linkage map
Gene expression regulation
We can design and customize data analysis strategy according to actual needs.
Strict data analysis process and strict data quality control ensure accurate and reliable data analysis results.
Customized charts can be used directly for publication.
CD Genomics provides RRBS data analysis service. Based on the promoter and CpG island regions, we can achieve accurate positioning of methylation sites with single-base resolution, quickly and accurately find differential sites, and achieve efficient analysis of DMR and related gene functional analysis. CD Genomics ensures the reliability of data analysis through strict data quality control.
|Data preprocessing||Data quality control|
|Remove low quality reads|
|Map to reference genome||Annotation analysis|
|Unique comparison rate|
|Reads distribution statistics|
|Methylation site calling||Analysis of methylation sites|
|Calculation of methylation level|
|Identification of differentially methylated regions (DMRs)|
|Differential methylation analysis||Related gene analysis|
|GO enrichment analysis of related genes|
|KEGG pathway enrichment analysis of related genes|
If you have any omics sequencing data, such as RNA sequencing data, we can provide multi-omics association analysis. For any custom data analysis request, please contact our technical support for evaluation. We will design the most suitable analysis strategy for you. For more information, please click online inquiry.
It takes about one to two weeks from obtaining the original data to issuing the result report, but the specific analysis time is also related to the content of the analysis. If there is a need for multi-group joint analysis, a longer analysis cycle is required.
CD Genomics has successfully conducted reduced representation bisulfite sequencing data analysis on a variety of species, combined with rich project experience, strict data quality control and professional analysis process, to ensure that the project is carried out accurately and quickly. Please contact us for more information and a detailed quote. We look forward to working with you!