CD Genomics uses bioinformatics to provide MeRIP-seq data analysis to global researchers, helping to conduct a comprehensive study of the RNA post-transcription methylation modification map. In addition, we also provide personalized analysis and chart making services.
Introduction to MeRIP-seq Analysis
RNA modification is widespread at the transcriptome level, and is a reversible chemical modification, an apparent modification in a state of dynamic regulation. This process is involved in all aspects of biological processes, including but not limited to cell differentiation decisions, sex decisions, stress responses, and m6A (N6-methyladenosine) modification (a widely studied RNA modification). The m6A modification is a highly conserved RNA modification between different species. It has been confirmed to exist in mammals, plants, yeast and other organisms. Among the known m6A modification studies, m6A is the most widespread RNA modification in both mRNA and non-coding RNA.
Methylated RNA ImmunoPrecipitation with high throughput Sequencing (meRIP-seq) is a sequencing technology that combines high-throughput sequencing with m6A RNA immunoprecipitation. The principle of MeRIP-seq, or the m6A-seq, is to specifically recognize m6A modified antibodies, co-immunoprecipitate m6A modified RNA fragments in cells, and perform high-throughput sequencing on the precipitated RNA fragments. By analyzing the sequencing data by bioinformatics, we can detect the modification of m6A site at the level of full transcriptome, and compare the differences of methylation modification patterns among different tissues, cells and samples. This method can be used to study the relationship between transcriptional regulation, mRNA modification, transcription and translation.
Fig 1. RNA N6-methyladenosine sequencing reveals a homogeneous m6A decoration in human T2D islets. (Jesus D F D ,et al. 2019)
Application Field
Cell Differentiation
Biological development
Disease occurrence and development research
Cancer occurrence and metastasis
RNA modification can regulate the stability, localization, transport, splicing and translation of RNA at the post-transcriptional level, so MeRIP is also helpful to the study of biological functions and mechanisms such as transcription regulation, embryonic development, lipid metabolism, circular RNA translation and DNA damage repair.
Advantages of CD Genomics
Strict data analysis process and strict data quality control ensure accurate and reliable data analysis results.
Customized charts can be used directly for article writing.
The data analysis plan can be customized according to actual needs.
Fast analysis cycle can speed up your research progress.
CD Genomics Data Analysis Pipeline
Bioinformatic Analysis Content
CD Genomics MeRIP sequencing analysis uses motif analysis to determine the specificity of immunoprecipitation and the credibility of the analysis, efficiently predict related genes, perform functional enrichment analysis, and construct m6A modification maps.
| Data preprocessing |
Data quality control |
| Data statistics |
| Remove adapter |
| Remove low quality reads |
| Map to reference genome |
Comparison rate |
| Unique comparison rate |
| Sequencing depth |
| The only comparison of reads distribution statistics |
| Peak calling |
Analysis of nucleosome and transcription factor binding sites |
| Peak position on chromosome |
| Peak length distribution |
| The distribution of peak on each genetic element |
| Motif analysis |
| Differential peak analysis |
Difference peak analysis |
| Difference peak gene annotation |
| GO enrichment analysis of related genes |
| KEGG pathway enrichment analysis of related genes |
For other omics sequencing data, such as RNA sequencing data, we can provide joint analysis of RNA-Seq and MeRIP data to analyze the regulatory mechanism of m6A. In addition, we also provide other personalized analysis and chart customization services. For more information, please click online inquiry.
How It Works
CD Genomics is a high-tech company specializing in multiomic data analysis. We provide services such as project design, data analysis, and database construction. With a focus on developing breakthrough products and services, we are a pioneer in the biotechnology industry, serving researchers and partners worldwide.
CD Genomics has successfully conducted MeRIP sequencing data analysis on a variety of samples. If you are interested in our services, please feel free to get in touch for more detailed information, and a CD Genomics representative is ready to answer your questions and get a complete understanding of your needs.
Reference
- Jesus D F D ,et al. m6A mRNA Methylation Regulates Human β-Cell Biology in Physiological States and in Type 2 Diabetes[J]. Nature Metabolism. 2019,1, 765–774.
