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MeRIP-seq Analysis

MeRIP-seq Analysis Online Inquiry

CD Genomics uses bioinformatics to provide MeRIP-seq data analysis to global researchers, helping to conduct a comprehensive study of the RNA post-transcription methylation modification map. In addition, we also provide personalized analysis and chart making services.

Introduction to MeRIP-seq Analysis

RNA modification is widespread at the transcriptome level, and is a reversible chemical modification, an apparent modification in a state of dynamic regulation. This process is involved in all aspects of biological processes, including but not limited to cell differentiation decisions, sex decisions, stress responses, and m6A (N6-methyladenosine) modification (a widely studied RNA modification). The m6A modification is a highly conserved RNA modification between different species. It has been confirmed to exist in mammals, plants, yeast and other organisms. Among the known m6A modification studies, m6A is the most widespread RNA modification in both mRNA and non-coding RNA.

Methylated RNA ImmunoPrecipitation with high throughput Sequencing (meRIP-seq) is a sequencing technology that combines high-throughput sequencing with m6A RNA immunoprecipitation. The principle of MeRIP-seq, or the m6A-seq, is to specifically recognize m6A modified antibodies, co-immunoprecipitate m6A modified RNA fragments in cells, and perform high-throughput sequencing on the precipitated RNA fragments. By analyzing the sequencing data by bioinformatics, we can detect the modification of m6A site at the level of full transcriptome, and compare the differences of methylation modification patterns among different tissues, cells and samples. This method can be used to study the relationship between transcriptional regulation, mRNA modification, transcription and translation.

RNA N6-methyladenosine sequencing reveals a homogeneous m6A decoration in human T2D islets.Fig 1. RNA N6-methyladenosine sequencing reveals a homogeneous m6A decoration in human T2D islets. (Jesus D F D ,et al. 2019)

Application Field

Cell Differentiation

Biological development

Disease occurrence and development research

Cancer occurrence and metastasis

RNA modification can regulate the stability, localization, transport, splicing and translation of RNA at the post-transcriptional level, so MeRIP is also helpful to the study of biological functions and mechanisms such as transcription regulation, embryonic development, lipid metabolism, circular RNA translation and DNA damage repair.

Advantages of CD Genomics

Strict data analysis process and strict data quality control ensure accurate and reliable data analysis results.

Customized charts can be used directly for article writing.

The data analysis plan can be customized according to actual needs.

Fast analysis cycle can speed up your research progress.

CD Genomics Data Analysis Pipeline

 CD Genomics MeRIP-seq data analysis pipeline - CD Genomics.

Bioinformatic Analysis Content

CD Genomics MeRIP sequencing analysis uses motif analysis to determine the specificity of immunoprecipitation and the credibility of the analysis, efficiently predict related genes, perform functional enrichment analysis, and construct m6A modification maps.

Data preprocessing Data quality control
Data statistics
Remove adapter
Remove low quality reads
Map to reference genome Comparison rate
Unique comparison rate
Sequencing depth
The only comparison of reads distribution statistics
Peak calling Analysis of nucleosome and transcription factor binding sites
Peak position on chromosome
Peak length distribution
The distribution of peak on each genetic element
Motif analysis
Differential peak analysis Difference peak analysis
Difference peak gene annotation
GO enrichment analysis of related genes
KEGG pathway enrichment analysis of related genes

For other omics sequencing data, such as RNA sequencing data, we can provide joint analysis of RNA-Seq and MeRIP data to analyze the regulatory mechanism of m6A. In addition, we also provide other personalized analysis and chart customization services. For more information, please click online inquiry.

Turnaround Time

MeRIP-seq data analysis turnaround time - CD Genomics.

About one to two weeks from obtaining the original data to issuing the result report, it's also related to the content of the project, and depending on the number of samples and the quality and size of the sample data. If there is a need for multi-group joint analysis, a longer analysis cycle is required.

CD Genomics has successfully conducted MeRIP sequencing data analysis on a variety of samples. If you are interested in our services, please feel free to get in touch for more detailed information, and a CD Genomics representative is ready to answer your questions and get a complete understanding of your needs.

Reference

  1. Jesus D F D ,et al. m6A mRNA Methylation Regulates Human β-Cell Biology in Physiological States and in Type 2 Diabetes[J]. Nature Metabolism. 2019,1, 765–774.
* For Research Use Only. Not for use in diagnostic procedures.
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