CD Genomics as one of the providers of mitochondrial genome analysis, We use the method of bioinformatics analysis to study mtDNA mutation sites and heterogeneity to help reveal the pathogenesis of mitochondrial-related diseases. Our unique data analysis skills and appropriate software tools can meet customers' personalized laboratory data analysis needs, and provide you with easy-to-interpret data analysis reports.
Mitochondria are a very important organelle in eukaryotic cells, and are the places where cells undergo oxidative phosphorylation. Mitochondria have their own DNA and genetic system, which are closely related to biological evolution and cell origin. The whole mitochondrial genome also has unique advantages in the study of biological evolution. Since mitochondria have a long-term evolutionary history of symbiosis with eukaryotes, their genome contains key information reflecting the evolution of species. At the same time, compared with the nuclear genome, the mitochondrial genome is small, and it is easy to conduct horizontal comparative analysis of a large number of species, which is beneficial to the study of biological evolution.
Mitochondrial genome data is widely used in systems and evolutionary biology, population genetics and conservation biology. With the development of sequencing technology and the reduction of costs, as well as the development of bioinformatics, a large number of mitochondrial genome sequences have been sequenced. The content of mitochondrial bioinformatics analysis mainly includes assembly, annotation, and structure drawing.
Fig 1. Characteristics of mtDNA variants. (Y Wang, M Picard, et al. 2016)
Classification and identification of species.
Research on genetics and molecular phylogeny of animal populations.
Mitochondrial DNA sequence variation is used as a marker for population genetics and phylogeny research.
Analysis of genetic variation in geographic populations.
Human identification, forensic medicine.
Detect common and rare mitochondrial point mutations and deletions.
Rapid analysis of mitochondrial DNA (mtDNA) and nuclear DNA (nDNA).
Accurately and sensitively measure heterogeneity.
Analysis of the highly variable region of the D-loop and/or the entire mitochondrial genome.
Fig 2. Pipeline of mitochondrial genome analysis.
Common NGS analysis methods for mitochondrial disease-related mutations include whole exome sequencing and targeted gene sequencing. For whole exome sequencing and targeted gene sequencing data, we provide standard biological information analysis and advanced analysis.
|Standard analysis||Sequencing data statistics|
|Remove adapter sequence and low-quality sequence|
|Align with mitochondrial reference sequence|
|Statistics sequencing depth and coverage|
|SNP / Indel detection|
|Annotation and statistics (including frequency database mtDB, OMIM, MitoMap, MitoIMPACT database)|
|Heterogeneity detection, annotation and statistics|
|Mitochondrial Circos diagram analysis|
|Advanced analysis||Mutation site harmfulness screening|
|Shared mutation screening|
|Site significance association analysis|
|Genetic significance analysis|
For Mitochondrial Genome advanced analysis, the sample size of the original data needs to meet a certain number of requirements before relevant analysis can be carried out. In addition, for data analysis, if you have other personalized requirements, we will provide appropriate biological information analysis content according to your needs. For analysis content, price, cycle, if you have any questions, please click online inquiry.
CD Genomics is a professional bioinformatics service provider with years of experience in NGS and long read sequencing (PacBio SMRT and Oxford Nanopore platforms) data analysis, integrated analysis services, database construction and other bioinformatics solutions.
Bioinformatics-Analysis, a division of CD Genomics, provides mitochondrial genome analysis according to customer’s requirements. It has been engaged in mitochondrial genome sequencing for a long time. Through research on various diseases, animals and plants, we have accumulated and solved various problems encountered in mitochondrial retrieval, such as gene rearrangement, repetitive sequences, high GC and other structures. If you are interested in our services, please contact us for more detailed information.