ChIP-seq Analysis

ChIP-seq Analysis

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As a leading provider of ChIP-Seq data analysis, CD Genomics uses bioinformatics to help you decode the mystery of protein and DNA binding. Our unique data analysis skill set combined with the most advanced software tools will meet clients' needs for personalized laboratory data analysis and provide the easy-to-interpret data analysis reports.

Introduction of ChIP-Seq Analysis

Chromatin Immunoprecipitation (ChIP), also known as the binding site analysis, is a powerful tool for studying the interaction of proteins and DNA in vivo, and is usually used for the study of transcription factor binding sites or histone-specific modification sites., Combining ChIP with the next generation sequencing technology, ChIP-Seq can efficiently detect DNA regions that interact with histones and transcription factors in the whole genome.

The principle of ChIP-Seq sequencing is to first specifically enrich the DNA fragments bound by the target protein by ChIP technology, purify the DNA fragments bound to the protein and construct a high-throughput sequencing library, and then perform sequencing. Millions of sequence tags obtained by sequencing are accurately aligned to the genome by bioinformatics methods, and the DNA region information that interacts with histones and transcription factors in the whole genome is obtained. It helps to gain insight into the regulation of protein expression by genes and the functional structure of chromosomes.ChIP-Seq related research has been widely used in human, mouse, yeast, rice, arabidopsis and other model species.

ChIP-Seq sequencing workflow - CD Genomics.Fig 1. ChIP-Seq sequencing workflow.

Application Field

ChIP-Seq data analysis can be used for the following research, but it is not limited to the following research.

Enhancer study

Transcription factor research

Histone modification

Nucleosome positioning

Advantages of CD Genomics

An experienced analytical team

Standardized analysis process

Strict data quality control

Reliable analysis results

Fast analysis cycle

CD Genomics Data Analysis Pipeline

CD Genomics ChIP-Seq data analysis pipeline - CD Genomics.

Bioinformatic Analysis Content

CD Genomics has a comprehensive, efficient, and highly accurate ChIP-Seq data analysis pipeline to provide our clients with fast, efficient and comprehensive analysis content.

Data preprocessing Data quality control
Data statistics
Remove adapter
Remove low quality reads
Map to reference genome Comparison rate
Unique comparison rate
Sequencing depth
Peak calling Peak length distribution statistics
Peak depth statistics
Peak annotation Distribution of peak on gene functional elements
Peak related gene analysis
GO enrichment analysis of related genes
KEGG pathway enrichment analysis of related genes
Difference analysis Distribution of genetic elements of differentially expressed peak related genes
GO enrichment analysis of differentially expressed peak related genes
KEGG pathway enrichment analysis of differentially expressed peak related genes

For ChIP-Seq data analysis, if you have other sequencing data, such as RNA sequencing data, we can provide you with correlation analysis of ChIP-Seq and RNA-Seq differentially expressed genes (DEGs). In addition, if you have any other personalized analysis needs, please contact us so that we can develop a biological information analysis strategy tailored for you. For any questions on analysis content, price, or cycle, please click online inquiry.

How It Works

CD Genomics is a high-tech company specializing in multiomic data analysis. We provide services such as project design, data analysis, and database construction. With a focus on developing breakthrough products and services, we are a pioneer in the biotechnology industry, serving researchers and partners worldwide.

How It Works

CD Genomics has over a decade of experience in ChIP-Seq data analysis. We can help with your project from experimental data, through all analyses and to result report at competitive rates. If you have any questions about how we can help you, please get in touch. We look forward to working with you!

* For Research Use Only. Not for use in diagnostic procedures.
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