As a leading provider of ChIP-Seq data analysis, CD Genomics uses bioinformatics to help you decode the mystery of protein and DNA binding. Our unique data analysis skill set combined with the most advanced software tools will meet clients' needs for personalized laboratory data analysis and provide the easy-to-interpret data analysis reports.
Chromatin Immunoprecipitation (ChIP), also known as the binding site analysis, is a powerful tool for studying the interaction of proteins and DNA in vivo, and is usually used for the study of transcription factor binding sites or histone-specific modification sites., Combining ChIP with the next generation sequencing technology, ChIP-Seq can efficiently detect DNA regions that interact with histones and transcription factors in the whole genome.
The principle of ChIP-Seq sequencing is to first specifically enrich the DNA fragments bound by the target protein by ChIP technology, purify the DNA fragments bound to the protein and construct a high-throughput sequencing library, and then perform sequencing. Millions of sequence tags obtained by sequencing are accurately aligned to the genome by bioinformatics methods, and the DNA region information that interacts with histones and transcription factors in the whole genome is obtained. It helps to gain insight into the regulation of protein expression by genes and the functional structure of chromosomes.ChIP-Seq related research has been widely used in human, mouse, yeast, rice, arabidopsis and other model species.
Fig 1. ChIP-Seq sequencing workflow.
ChIP-Seq data analysis can be used for the following research, but it is not limited to the following research.
Transcription factor research
An experienced analytical team
Standardized analysis process
Strict data quality control
Reliable analysis results
Fast analysis cycle
CD Genomics has a comprehensive, efficient, and highly accurate ChIP-Seq data analysis pipeline to provide our clients with fast, efficient and comprehensive analysis content.
|Data preprocessing||Data quality control|
|Remove low quality reads|
|Map to reference genome||Comparison rate|
|Unique comparison rate|
|Peak calling||Peak length distribution statistics|
|Peak depth statistics|
|Peak annotation||Distribution of peak on gene functional elements|
|Peak related gene analysis|
|GO enrichment analysis of related genes|
|KEGG pathway enrichment analysis of related genes|
|Difference analysis||Distribution of genetic elements of differentially expressed peak related genes|
|GO enrichment analysis of differentially expressed peak related genes|
|KEGG pathway enrichment analysis of differentially expressed peak related genes|
For ChIP-Seq data analysis, if you have other sequencing data, such as RNA sequencing data, we can provide you with correlation analysis of ChIP-Seq and RNA-Seq differentially expressed genes (DEGs). In addition, if you have any other personalized analysis needs, please contact us so that we can develop a biological information analysis strategy tailored for you. For any questions on analysis content, price, or cycle, please click online inquiry.
Depending on the content of the project, number of samples, and the quality and size of the sample data, we will take approximately one to two weeks to complete the analysis. For more information, please contact us.
CD Genomics has over a decade of experience in ChIP-Seq data analysis. We can help with your project from experimental data, through all analyses and to result report at competitive rates. If you have any questions about how we can help you, please get in touch. We look forward to working with you!