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CRISPR-Based Functional Gene Analysis

CRISPR-Based Functional Gene Analysis Online Inquiry

CD Genomics, as one of the most competitive service providers in sequencing and data analysis, we use CRISPR technologies and advanced bioinformatics to help you explore the genetic information and decipher the essence of life. Our skills and experience in data analysis can meet customers' personalized data analysis needs.

Introduction of CRISPR-Based Functional Gene Analysis

Core cellular procedures are underpinned by important gene functions. To recognize how cell physiology is regulated and to facilitate drug advancement, questioning the interactions between crucial gene functions is critical. Yet, to explicate their connections, few strategies can evaluate essential gene function in vivo. In order to investigate vital genes, neither gene-deletion libraries nor saturating transposon mutagenesis can be applied, as cells cannot sustain without their mechanisms. Numerous high-throughput techniques have been employed to define or perturb crucial genes in eukaryotes, such as disrupting the 30 UTR of mRNAs, CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 gene editing, and The CRISPR screen has appeared lately as a thrilling new strategy to the genome level profile of gene essentiality. Single-guide RNAs (sgRNA) direct Cas9 nucleases within the CRISPR system to stimulate double-strand breaks (DSB) in targeted genomic areas. The only study that evaluated key genes in bacteria used antisense RNA knockdowns to evaluate for antibiotic sensitivities, a method of limited usefulness due to differential efficacy.

The advancement of CRISPR screen technology has made it possible to determine the essentiality of genes systematically and reliably under various conditions. CRISPR's high-quality gene essentiality profiles could allow for a better comparison between the methodologies of forecasting of significance and better classification of the various characteristics of the essential genes. Not only do these characteristics enable a better understanding of the CRISPR screen data, but they can also assist to prioritize CRISPR screen leads.

The Bioinformatics Analysis That We Can Provide

CD Genomics provides CRISPR systems that allow genome-scale functional screening in a cost-effective manner, associated with the lentiviral delivery mechanism. sgRNAs targeting candidate genes are synthesized on CRISPR screens, and viral integration allows reading through next-generation sequencing. The relative amplitudes of each integrated sgRNA are contrasted between various situations and pertaining to the impact of its sgRNAs on cell growth, the significance of the sgRNA target gene is implied. And enhance the performance of the outcome of the CRISPR screen.

CD Genomics has formed a cost-effective, high-throughput tactic for CRISPR-based functional gene analysis. We offer effective and straightforward essential gene interpretation based on years of experience in genome sequencing and data analysis.  Our talented bioinformaticians will work closely with you to help you reach the goal of your studies with a completely customized scheme.

Advantages of CRISPR-Based Functional Gene Analysis

Relatively straightforward, yet very effective

Compared to gene targeting innovations, less time was needed to adjust target genes

Can classify the most suitable sequences for designing guide RNAs

Experimental circumstances can be optimized to allow very robust systems to ensure the preferred mutation is successfully introduced.

CD Genomics Pooled CRISPR Screen Analysis Pipeline

Pooled CRISPR Screen Analysis Pipeline

Bioinformatics Analysis Content

Analysis/Pipeline Details
Raw Data QC Gives a set of SNPs and individuals filtered out from
Reference Alignment Classify similarity regions that may be the result of functional, structural, or evolutionary connections between the sequences
Validation of CRISPR Libraries Offer the CRISPR libraries themselves with validation using high-throughput sequencing
Analysis of Target Loci For phylogenetic and barcoding evaluation, results can be employed

How It Works

CD Genomics is a professional bioinformatics service provider with years of experience in NGS and long read sequencing (PacBio SMRT and Oxford Nanopore platforms) data analysis, integrated analysis services, database construction and other bioinformatics solutions.

How It Works

CD Genomics has over a decade of experience in sequencing and analysis. We can help you with your project from experimental data, through all analyses to intact report at competitive rates. If you have any questions about how we can help you, please get in touch. We look forward to working with you!

References

  1. Arroyo JD, Jourdain AA, Calvo SE, et al. A genome-wide CRISPR death screen identifies genes essential for oxidative phosphorylation. Cell metabolism. 2016, 24(6).
  2. Jiang P, Wang H, Li W, et al. Network analysis of gene essentiality in functional genomics experiments. Genome biology. 2015, 16(1).
  3. Peters JM, Colavin A, Shi H, et al. A comprehensive, CRISPR-based functional analysis of essential genes in bacteria. Cell. 2016, 165(6).
* For Research Use Only. Not for use in diagnostic procedures.
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