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Whole Genome Bisulfite Methylation Analysis

Whole Genome Bisulfite Methylation Analysis Online Inquiry

CD Genomics provides whole genome bisulfite methylation sequencing data analysis. We use bioinformatics and a high-quality data analysis platform to analyze genomic methylation comprehensively and accurately. Our unique data analysis skills can also meet customers' personalized data analysis needs.

Introduction of Whole Genome Bisulfite Methylation

Whole Genome Bisulfite Sequencing (WGBS) is a combination of bisulfite treatment method and high-throughput sequencing platform. WGBS can achieve single-base resolution, accurately analyzing the methylation status of each cytosine and obtaining the genome-wide methylation C site, which is the gold standard for methylation sequencing.

Whole genome bisulfite methylation sequencing data analysis is using whole-genome methylation sequencing data, combined with bioinformatics data analysis, to obtain methylation level data of all C sites within the whole genome, thereby constructing a refined whole genome DNA methylation profile. The low-cost, high-efficiency and high-precision mapping of genome-wide DNA methylation levels through bioinformatics is of great significance for the study of the spatiotemporal specificity of epigenetics.

Optimizing the DNA methylation resolution according to a biological context. The most widely used strategy for integrating DNA methylation with gene regulation is to average CpG methylation signal throughout wide loci. Fig 1. Optimizing the DNA methylation resolution according to a biological context. The most widely used strategy for integrating DNA methylation with gene regulation is to average CpG methylation signal throughout wide loci. (Tirado-Magallanes R , et al. 2017)

Application Field

Methylation is essential for life activities. It is one of the methods of gene regulation, and a hot spot in epigenetics research. Whole genome bisulfite methylation sequencing data analysis can be used for but is not limited to the following research.

Gene expression regulation.

Disease development and regulation.

Regulation of growth and development of animals and plants.

Environment and epigenetics.

Molecular breeding.

Genetic imprinting.

Advantages

Whole genome bisulfite methylation has the advantages of high efficiency, precision and value, and has been widely used in human, animal and plant research.

The methylation site with single base resolution and the differential methylation region can be located accurately and efficiently.

The effects of different treatments on genome-wide methylation can be analyzed comprehensively.

Find the differential methylation region quickly and accurately.

CD Genomics Data Analysis Pipeline

CD Genomics whole genome bisulfite methylation sequencing data analysis Pipeline - CD Genomics.

Bioinformatics Analysis Content

The whole genome bisulfite methylation data analysis provided by CD Genomics is based on the whole genome level, and realizes the accurate positioning of methylation sites with single-base resolution, finds the difference sites quickly and accurately, and realizes the functional analysis of efficient analysis of DMR and related genes. And ensure the reliability of data analysis through strict data quality control.

Sequencing data quality assessment

Remove adapters

Filter low-quality reads to ensure data quality

Map to reference genome

Comparison analysis

Coverage analysis

Methylation site calling

Analysis of methylation sites

Methylation distribution

Distribution of methylation on genomes analysis

Distribution of methylation on chromosomes analysis

Distribution of methylation on functional elements

Differential methylation analysis

Gene-based DMRs analysis.

DMRs Analysis based on miRNA.

Repetitive sequence DMRs analysis

Related gene analysis

GO annotation and enrichment analysis

KEGG annotation and enrichment analysis

For whole genome bisulfite methylation data analysis,if you have other needs, such as multi-sample analysis, we will provide appropriate biological information analysis content according to your needs, please feel free to contact us for details.

Turnaround Time

About one to two weeks, it's related to the content of the project, For more information, please feel free to get in touch.

CD Genomics has successfully conducted whole genome bisulfite methylation data analysis on a variety of samples. Combined with rich project experience, strict data quality control and professional analysis process, to ensure that the project is carried out accurately and quickly. Please contact us for more information and a detailed quote.

Reference

  1. Tirado-Magallanes R , et al. Whole genome DNA methylation: beyond genes silencing. Oncotarget, 2017, 8(3).
* For Research Use Only. Not for use in diagnostic procedures.
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