CD Genomics uses bioinformatics to provide qualitative proteome data analysis and help you identify the protein composition in your samples quickly and accurately. Using our high-quality data analysis platform, we can also provide customers with customized analysis services.
Introduction to Qualitative Proteome Analysis
Protein is the material basis of life, and the first step in the study of protein is the qualitative analysis of protein. Mass spectrometry technology is used to separate, purify, and enrich cells, tissues or body fluids. All or specific proteins in the protein gel/liquid type sample is then identified before the type of protein in a specific environment is fully understood to provide the basis for proteomics research.
With the development of sequencing technology, the research on protein characteristics is often carried out on a large-scale. As a result, the amount of data obtained has also increased significantly. Therefore, it is necessary to use bioinformatics to fully mine the relevant information. In addition to the use of protein analysis software to predict various parameters, the isoelectric point, molecular weight, primary structure, and secondary structure are verified and analyzed by instruments and equipment. After analyzing the protein in an all-round way combined with the instrument and analysis software, the function and mechanism of the protein are further inferred. We can provide intuitive protein identification results for research on the occurrence and development of diseases and the growth and development of animals and plants. The protein identification and analysis products can be divided into the following two products according to the research object and content: protein full spectrum analysis and glue dot/strip identification.
Fig 1.Flow chart of protein mass spectrometry identification experiment. (Zhang Y, et al. 2015)
Application Field
Protein identification analysis can be applied to but not limited to the following fields:
Identification of the protein contained in the sample
Proteomics database construction
Research on the regulation of key proteins in the development of disease
Study on the interaction of key proteins before and after drug treatment
Advantages of CD Genomics
An experienced analytical team
Standardized analysis process
Strict data quality control
Reliable analysis results
Wide analysis range, analysis of protein data of all species
Fast analysis cycle
CD Genomics Data Analysis Pipeline
Bioinformatic Analysis Content
CD Genomics searches the corresponding protein database based on the original data obtained by mass spectrometry, and then performs protein identification based on the results of the database search. At the same time, we can analyze the mass tolerance distribution of peptides, proteins and precursor ions to evaluate the quality of mass spectrometry data, then annotate the identified proteins in conventional functional databases, including COG, GO and KEGG databases. The following analysis content is suitable for full spectrum analysis and strip identification analysis.
|
Standard analysis content |
Data output statistics |
| Protein database search |
| Preliminary protein identification |
| Quality control, data filtering |
| Protein identification results |
| Protein GO analysis |
| Protein COG analysis |
| Protein pathway analysis |
| Advanced analysis content |
Relative abundance quantification of single sample protein |
For qualitative proteome data analysis, if you have other personalized analysis needs, we will provide appropriate biological information analysis content according to your needs. For analysis content, price, cycle, if you have any questions, please click online inquiry.
CD Genomics provides general analysis and customized analysis of qualitative proteome data analysis. Experienced teams of scientists, researchers, and technicians, we provide fast turnaround, high-quality data reports at competitive prices for worldwide customers. Customers can contact our employees directly and we will respond promptly. If you are interested in our services, please contact us for more detailed information.
Reference
- Zhang Y, et al. Tissue-Based Proteogenomics Reveals that Human Testis Endows Plentiful Missing Proteins.[J]. Journal of Proteome Research, 2015:3583-3594.
