Amino Acids and Derivatives Metabolomics Data Analysis

In addition to the 20 amino acids that make up the proteins of the animal body and its products, 200 amino acid derivatives have been identified in nature and play an important role in many metabolic pathways as substrates and regulators. Amino acid derivatives are substances formed from amino acids through a series of reactions, such as the combined deamidation of amino acids to synthesize amino acid derivatives. Various substances in the human body, such as adrenaline and thyroid hormones, are amino acid derivatives.

Introduction of Targeted Metabolomics Analysis Services for Amino Acids and Their Derivatives

Amino acids are important nutrients for the maintenance of the living organism and are classified into essential and non-essential amino acids in terms of their nutritional structure. Amino acids play a direct or indirect role in regulating the health of the body. Diseases caused by amino acid imbalance include diabetes, immune system disorders, cardiovascular diseases, neurological diseases, infectious diseases, and other types of diseases. The specific services that our targeted metabolomics analysis service for amino acids and their derivatives can provide are listed below.

• Identification of differential amino acids and their derivatives.
• Identification of amino acid metabolic pathways.
• Correlation analysis of amino acid biosynthetic gene expression and metabolites of synthetic pathways.
• Full profile amino acid analysis.

Figure 1. Merged targeted quantification and untargeted profiling workflow for comprehensive assessment of acylcarnitine and amino acid metabolism in clinically relevant biofluids and tissue samples. (Benito S, et al., 2016)

Workflow of Targeted Metabolomics Analysis Services for Amino Acids and Their Derivatives

Deliverables

We can provide the following analysis results, including but not limited to.

• Volcano map reflecting amino acid changes.
• Pathway enrichment analysis of amino acid metabolic pathways.
• Heat map reflecting significant Spearman correlation between differential metabolites.
• Box plot reflecting metabolite differences.

How Can We Help You

Choosing our targeted metabolomics analysis services for amino acids and their derivatives can help you solve the following problems.

• We are able to use targeted metabolomic analysis of amino acids and their derivatives to identify significantly altered amino acids and their derivatives to further explore the pathogenesis of the disease.
• We are able to screen for differential metabolites using targeted metabolomics analysis of amino acids and their derivatives to help our customers find disease markers.

Can CD Genomics help me generate raw data?

Yes, we can. With advanced instrumentation, a high-coverage self-built database, and experienced metabolomics experts, we can provide you with amino acids and their derivatives detection services. We are able to perform the quantitative and qualitative analysis of the following amino acids and their derivatives using LC-MS/MS and GC-MS/MS techniques.

How should I prepare and send my samples?

If you require our amino acids and their derivatives detection services, please send samples as requested in the form below and contact us. A minimum of 6 biological replicates per group is required for cellular/microbial samples. A minimum of 10 biological replicates per group is required for model animal/plant samples. Clinical samples generally require a minimum of 50 biological replicates per group.

Reference

1. Benito S, et al. LC-QTOF-MS-based targeted metabolomics of arginine-creatine metabolic pathway-related compounds in plasma: application to identify potential biomarkers in pediatric chronic kidney disease. Anal Bioanal Chem. 2016 Jan; 408(3): 747-60.